EZH2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

EZH2 Monoklonal Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F10A12

Kat-Nr. : 66476-1-PBS

Synonyme

1F10A12, EC:2.1.1.356, Enhancer of zeste homolog 2, ENX 1, ENX-1

---

• 

  WB analysis of HEK-293 using 66476-1-Ig (same clone as 66476-1-PBS)

  WB result of EZH2 antibody (66476-1-Ig; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EZH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

  Various cell lines were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66476-1-Ig (same clone as 66476-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66476-1-Ig (same clone as 66476-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  WB analysis of ROS1728 using 66476-1-Ig (same clone as 66476-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66476-1-Ig (same clone as 66476-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  WB analysis of 4T1 using 66476-1-Ig (same clone as 66476-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66476-1-Ig (same clone as 66476-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66476-1-Ig (EZH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 66476-1-Ig (same clone as 66476-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EZH2 (66476-1-Ig, Clone:1F10A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66476-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66476-1-PBS bindet in WB, FC (Intra), Indirect ELISA EZH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: EZH2 fusion protein Ag16789
• Vollständiger Name: enhancer of zeste homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 751 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-102 kDa
• GenBank-Zugangsnummer: BC010858
• Gene symbol: EZH2
• Gene ID (NCBI): 2146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)