EZH2 Rekombinanter Antikörper

EZH2 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

242309F11

Kat-Nr. : 84824-2-RR

Synonyme

242309F11, EC:2.1.1.356, Enhancer of zeste homolog 2, ENX 1, ENX-1

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  WB analysis using 84824-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84824-2-RR (EZH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 84824-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84824-2-RR (EZH2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 84824-2-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 84824-2-RR (EZH2 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 84824-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-2-RR, Clone: 242309F11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  ChIP experiment of NCCIT using 84824-2-RR

  Chromatin was prepared from NCCIT cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of EZH2 (84824-2-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 84824-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84824-2-RR against Human EZH2 were performed. The affinity constant is 2.73 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, C6-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in ChIP-qPCR: NCCIT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000
• CHImmunpräzipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84824-2-RR bindet in WB, IHC, IF/ICC, ELISA, ChIP-qPCR EZH2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EZH2 fusion protein Ag16789
• Vollständiger Name: enhancer of zeste homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 751 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-102 kDa
• GenBank-Zugangsnummer: BC010858
• Gene symbol: EZH2
• Gene ID (NCBI): 2146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)