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EZH2 Rekombinanter Antikörper

EZH2 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242309F9

Kat-Nr. : 84824-3-PBS

Synonyme

242309F9, EC:2.1.1.356, Enhancer of zeste homolog 2, ENX 1, ENX-1

Filter:
  • Western Blot (WB) analysis of various lysates using EZH2 Recombinant antibody (84824-3-RR)

    WB analysis using 84824-3-RR (same clone as 84824-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84824-3-RR (EZH2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84824-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EZH2 Recombinant antibody (84824-3-RR)

    IF Staining of HepG2 using 84824-3-RR (same clone as 84824-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-3-RR, Clone: 242309F9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84824-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84824-3-RR

    Affinity and Kinetic Characterization of 84824-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84824-3-RR against Human EZH2 were performed. The affinity constant is 4.00 nM.

Geprüfte Anwendungen

Produktinformation

84824-3-PBS bindet in WB, IF/ICC, Indirect ELISA EZH2 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen EZH2 fusion protein Ag16789
Vollständiger Name enhancer of zeste homolog 2 (Drosophila)
Berechnetes Molekulargewicht 751 aa, 86 kDa
Beobachtetes Molekulargewicht 90-102 kDa
GenBank-ZugangsnummerBC010858
Gene symbol EZH2
Gene ID (NCBI) 2146
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)