EZH2 Rekombinanter Antikörper

EZH2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242309F9

Kat-Nr. : 84824-3-RR

Synonyme

242309F9, EC:2.1.1.356, Enhancer of zeste homolog 2, ENX 1, ENX-1

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  WB analysis using 84824-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84824-3-RR (EZH2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 84824-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using EZH2 antibody (84824-3-RR, Clone: 242309F9 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84824-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84824-3-RR against Human EZH2 were performed. The affinity constant is 4.00 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84824-3-RR bindet in WB, IF/ICC, ELISA EZH2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: EZH2 fusion protein Ag16789
• Vollständiger Name: enhancer of zeste homolog 2 (Drosophila)
• Berechnetes Molekulargewicht: 751 aa, 86 kDa
• Beobachtetes Molekulargewicht: 90-102 kDa
• GenBank-Zugangsnummer: BC010858
• Gene symbol: EZH2
• Gene ID (NCBI): 2146
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EZH2 (enhancer of zeste homologue 2, also known as KMT6) is a member of Polycomb group (PcG) family and encodes a histone methyl transferase that has an essential role in promoting histone H3 lysine 27 trimethylation (H3K27me3) and epigenetic gene silencing. EZH2 is important for cell proliferation and inhibition of cell differentiation, and is implicated in cancer progression. Overexpression of EZH2 is a marker of advanced and metastatic disease in many solid tumors, including prostate and breast cancer. This antibody detected EZH2 protein as a single band with a molecular weight (MW) of 91-100 kDa in multiple cell lines. The phosphorylation may result in the higher molecular weight (calculated MW as 80-86 kDa). (20935635, 21367748)