Endoglin/CD105 Polyklonaler Antikörper

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• KD/KO Validated

Endoglin/CD105 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30730-1-AP

Synonyme

CD105, END, Endoglin, ENG, HHT1

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  WB analysis of HeLa using 30730-1-AP

  WB result of Endoglin/CD105 antibody (30730-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Endoglin/CD105 transfected HeLa cells.

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  WB analysis using 30730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30730-1-AP (Endoglin/CD105 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human renal cell carcinoma using 30730-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 30730-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 30730-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 30730-1-AP (Endoglin/CD105 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 30730-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30730-1-AP (ENG antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 30730-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30730-1-AP (ENG antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 30730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Endoglin/CD105 antibody (30730-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 30730-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Endoglin/CD105 antibody (30730-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:300-1:1200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30730-1-AP bindet in WB, IHC, IF-P, ELISA Endoglin/CD105 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Endoglin/CD105 fusion protein Eg0587
• Vollständiger Name: endoglin
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC014271
• Gene symbol: CD105
• Gene ID (NCBI): 2022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulfide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodeling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)