Endoglin/CD105 Monoklonaler Antikörper

Endoglin/CD105 Monoklonal Antikörper für FC, IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

CloneNo.

2D5E8

Kat-Nr. : 67075-1-Ig

Synonyme

CD105, END, endoglin, ENG, FLJ41744, HHT1, ORW, ORW1

---

Galerie der Validierungsdaten

• 

  WB analysis using 67075-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 67075-1-Ig

  HL-60 cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 67075-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67075-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human renal cell carcinoma using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens) Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

• 

  IHC staining of human tonsillitis using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 67075-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67075-1-Ig (Endoglin/CD105 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 67075-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 67075-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:10000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 67075-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human liver cancer using 67075-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using Endoglin/CD105 antibody (67075-1-Ig, Clone: 2D5E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of HUVEC using 67075-1-Ig

  1X10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human Endoglin/CD105 (67075-1-Ig, Clone:2D5E8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, humanes Plazenta-Gewebe, HUVEC-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Nierenzellkarzinomgewebe, humanes Plazenta-Gewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, humanes Leberkarzinomgewebe
• Erfolgreiche Detektion in FC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunhistochemie (IHC): IHC : 1:10000-1:40000
• Immunfluoreszenz (IF): IF : 1:5000-1:20000
• Durchflusszytometrie (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 2 publications below
• FC: See 2 publications below

Produktinformation

67075-1-Ig bindet in WB, IHC, IF, FC, ELISA Endoglin/CD105 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Endoglin/CD105 fusion protein Ag27837
• Vollständiger Name: endoglin
• Berechnetes Molekulargewicht: 71 kDa
• Beobachtetes Molekulargewicht: 95-100 kDa
• GenBank-Zugangsnummer: BC014271
• Gene symbol: ENG
• Gene ID (NCBI): 2022
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481)

Publikationen

Species	Application	Title
human	IF	Stem Cell Res Ther; Glioma-associated mesenchymal stem cells-mediated PD-L1 expression is attenuated by Ad5-Ki67/IL-15 in GBM treatment.; Authors - Qing Zhang
human	IHC,IF	Cell Tissue Res; A histological study of vascular wall resident stem cells in venous malformations; Authors - Hou-Fu Xia
mouse	FC	Dis Markers; Long Noncoding RNA SBF2-AS1 Promotes Abdominal Aortic Aneurysm Formation through the miRNA-520f-3p/SMARCD1 Axis; Authors - Baihe Han
rat	FC	Front Bioeng Biotechnol; Quiescence preconditioned nucleus pulposus stem cells alleviate intervertebral disc degeneration by enhancing cell survival via adaptive metabolism pattern in rats; Authors - Qi Chen