Endothelin 3 Polyklonaler Antikörper

Endothelin 3 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27871-1-PBS

Synonyme

EDN3, Endothelin-3, ET 3, ET3, ET-3

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  WB analysis using 27871-1-AP (same clone as 27871-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27871-1-AP (Endothelin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 27871-1-AP (same clone as 27871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

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  IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 27871-1-AP (same clone as 27871-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 27871-1-AP (EDN3 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 27871-1-AP (same clone as 27871-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Endothelin 3 antibody (27871-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 27871-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27871-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA Endothelin 3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Endothelin 3 fusion protein Ag27393
• Vollständiger Name: endothelin 3
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC053866
• Gene symbol: Endothelin 3
• Gene ID (NCBI): 1908
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Endothelin 3 (EDN3) is also named as ET-3 and Preproendothelin-3 (PPET3). Endothelin 3 belongs to the endothelin/sarafotoxin family. It reported downregulation of EDN3 as a result of methylation of its promoter in breast cancer, and EDN3 level was also decreased in cervical cancer (PMID: 36542159). ET-3 is the most abundant ET peptide in the rat brain, mainly localized to neurons and glia of the neostriatum, hypothalamic nuclei, hippocampus, and Purkinje cells of the cerebellum and medulla oblongata (PMID: 32589590).