Entactin Monoklonaler Antikörper

Entactin Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2A11G1

Rezensionen (2)

Kat-Nr. : 67310-1-Ig

Synonyme

Entactin, NID, NID 1, NID1, nidogen 1

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  WB analysis of mouse kidney using 67310-1-Ig

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67310-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 67310-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 67310-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 67310-1-Ig

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 67310-1-Ig

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 67310-1-Ig

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 67310-1-Ig (Entactin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 67310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 67310-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67310-1-Ig (Entactin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 67310-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Entactin antibody (67310-1-Ig, Clone: 2A11G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 67310-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using Entactin antibody (67310-1-Ig, Clone: 2A11G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, HeLa-Zellen, humanes Plazenta-Gewebe, Mausherzgewebe, Hausschwein-Herzgewebe, Rattenherzgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67310-1-Ig bindet in WB, IHC, ELISA Entactin und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Entactin fusion protein Ag4739
• Vollständiger Name: nidogen 1
• Berechnetes Molekulargewicht: 1247 aa, 136 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: BC045606
• Gene symbol: Entactin/NID
• Gene ID (NCBI): 4811
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Avelina (Verified Customer) (04-02-2025)

We titrated 5 different NID1 antibodies and this one performed the best. Current staining appears specific and relevant to what we find in literature.

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: NHP Placenta

FH

Jillian (Verified Customer) (03-27-2025)

We tested multiple NID1 antibodies and this was the cleanest and best looking one that we recieved

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Placenta