Entactin Rekombinanter Antikörper

Entactin Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

251203H4

Kat-Nr. : 86456-1-PBS

Synonyme

Ent, Nid1, NID-1, Nidogen-1

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  WB analysis using 86456-1-RR (same clone as 86456-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86456-1-RR (Entactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86456-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 86456-1-RR (same clone as 86456-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86456-1-RR (Entactin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86456-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse heart using 86456-1-RR (same clone as 86456-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Entactin antibody (86456-1-RR, Clone: 251203H4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86456-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

86456-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA Entactin und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Entactin fusion protein Eg3030
• Vollständiger Name: nidogen 1
• Berechnetes Molekulargewicht: 137 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_010917.3
• Gene symbol: Nid1
• Gene ID (NCBI): 18073
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nidogen 1 (Entactin), first identified from a basement membrane-secreting cell line and the murine EHS tumor, is together with perlecan, laminin, and collagen IV, a ubiquitous component of basement membranes (PMID: 19530222). Nidogen-1 is made by mesenchymal cells and is incorporated into the epithelial BM. It is a sulfated glycoprotein of 150 kDa with three globular domains, G1, G2 and G3, and contains binding sites for other ECM molecules (PMID: 10958695, 10671374).