EphA4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EphA4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21875-1-PBS

Synonyme

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  WB analysis using 21875-1-AP (same clone as 21875-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21875-1-AP (EphA4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

  IP result of anti-EphA4 (IP:21875-1-AP, 4ug; Detection:21875-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 21875-1-AP (same clone as 21875-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21875-1-AP (EphA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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  IF Staining of Neuron cells using 21875-1-AP (same clone as 21875-1-PBS)

  IF result of EphA4 antibody (21875-1-AP, 1:50) with culture neuron cells by Wilson Pak Kin Lou. This data was developed using the same antibody clone with 21875-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21875-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA EphA4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EphA4 fusion protein Ag16233
• Vollständiger Name: EPH receptor A4
• Berechnetes Molekulargewicht: 986 aa, 110 kDa
• Beobachtetes Molekulargewicht: 120 kDa
• GenBank-Zugangsnummer: BC026327
• Gene symbol: EPHA4
• Gene ID (NCBI): 2043
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EphA4 is a member of the Eph receptor tyrosine kinase family and has important functions in the developing and adult nervous system (PMID: 14697664). The Eph receptors comprise a large family of closely related transmembrane tyrosine kinases that actively signal when bound to their ephrin ligands. The Eph receptors are characterized by an extracellular region with a unique cysteine-rich motif extending over its amino-terminal half, followed by two fibronectin type III motifs (PMID: 9530499). They are divided into two sub-groups (EphA and EphB) based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands (PMID: 11114742). EphA4 is involved in commissure formation within the forebrain, axonal guidance in the corticospinal tract, regulation of the central pattern generator that provides normal locomotor function and axonal regeneration following spinal cord injury (PMID: 30061574). EphA4 has been implicated as a disease modifier of amyotrophic lateral sclerosis (ALS) (PMID: 22922411).