Ephrin B1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Ephrin B1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12999-1-PBS

Synonyme

EFL3, EFNB1, EPLG2, LERK2, EFL-3

---

• 

  WB analysis using 12999-1-AP (same clone as 12999-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12999-1-AP (Ephrin B1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12999-1-AP (same clone as 12999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 12999-1-AP (same clone as 12999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12999-1-AP (same clone as 12999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12999-1-AP (same clone as 12999-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12999-1-AP (Ephrin B1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12999-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

12999-1-PBS bindet in WB, IHC, Indirect ELISA Ephrin B1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Ephrin B1 fusion protein Ag3669
• Vollständiger Name: ephrin-B1
• Berechnetes Molekulargewicht: 346 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC016649
• Gene symbol: Ephrin B1
• Gene ID (NCBI): 1947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EFNB1 (Ephrin B1) is a member of the ephrin family, which are membrane-bound guidance cues that interact with Eph receptors, a family of receptor tyrosine kinases. EFNB1 is X-inactivated, and it has been proposed that in heterozygous females, patchy loss of ephrin B1 disturbs tissue boundary formation at the developing coronal suture (PMID: 34824583, 18627045). EFNB1 mainly plays a central part in cell adhesion, angiogenesis, and development of the nervous system (PMID: 30326247).