Exportin 4 Rekombinanter Antikörper

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• KD/KO Validated

Exportin 4 Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241443A5

Kat-Nr. : 84449-1-PBS

Synonyme

XPO4, 241443A5, Exp4, Exportin-4, KIAA1721

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  WB analysis using 84449-1-RR (same clone as 84449-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84449-1-RR (XPO4 antibody) at dilution of 1:8500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 84449-1-RR (same clone as 84449-1-PBS)

  WB result of Exportin 4 antibody (84449-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Exportin 4 transfected HEK-293 cells. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

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  IF Staining of K-562 using 84449-1-RR (same clone as 84449-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using Exportin 4 antibody (84449-1-RR, Clone: 241443A5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 84449-1-RR (same clone as 84449-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Exportin 4 Recombinant antibody (84449-1-RR, Clone:241443A5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84449-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84449-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84449-1-RR against Human Exportin 4 were performed. The affinity constant is 0.512 nM.

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Geprüfte Anwendungen

Produktinformation

84449-1-PBS bindet in WB, IF/ICC, FC (Intra), Indirect ELISA Exportin 4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Exportin 4 fusion protein Ag21845
• Vollständiger Name: exportin 4
• Berechnetes Molekulargewicht: 1151 aa, 130 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC113571
• Gene symbol: Exportin 4/XPO4
• Gene ID (NCBI): 64328
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

exportin 4 (XPO4) maps to chromosome 13q and belongs to the importin β family which acts as a bidirectional nuclear transport receptor. Decreased XPO4 expression has been shown to associate with hepatocellular carcinoma. A genome-wide copy number variation (CNV) association study in a small cohort of Malaysian subjects identified a CNV in XPO4 to be correlated with the progression of metabolic (dysfunction) associated fatty liver disease (MAFLD). (PMID: 34388518)