Ezrin Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

Ezrin Rekombinant Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

250065E1

Kat-Nr. : 85790-4-RR

Synonyme

EZR, VIL2, Villin-2

---

• 

  WB analysis using 85790-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85790-4-RR (Ezrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 85790-4-RR

  WB result of Ezrin antibody (85790-4-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ezrin transfected HeLa cells.

• 

  IP experiment of HeLa using 85790-4-RR

  IP result of anti-Ezrin (IP:85790-4-RR, 4ug; Detection:85790-4-RR 1:3000) with HeLa cells lysate 1280 ug.

• 

  IHC staining of Mouse small intestine using 85790-4-RR

  Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH6).

• 

  IHC staining of mouse stomach using 85790-4-RR

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 85790-4-RR (Ezrin antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

• 

  IF Staining of HeLa using 85790-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ezrin antibody (85790-4-RR, Clone: 250065E1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  Affinity and Kinetic Characterization of 85790-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85790-4-RR against Human Ezrin were performed. The affinity constant is 0.426 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, C6-Zellen, Jurkat-Zellen, MOLT-4-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85790-4-RR bindet in WB, IHC, IF/ICC, IP, ELISA Ezrin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Ezrin fusion protein Ag23530
• Vollständiger Name: ezrin
• Berechnetes Molekulargewicht: 69 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC013903
• Gene symbol: Ezrin
• Gene ID (NCBI): 7430
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ezrin is a member of the ERM (Ezrin, Radixin, Moesin) protein family and serves as a crucial linker between the cell membrane and the actin cytoskeleton. It plays significant roles in various cellular processes, including maintaining cell morphology, facilitating cell movement and adhesion, regulating signal transduction, and influencing apoptosis.