JAM-A/CD321 Polyklonaler Antikörper

JAM-A/CD321 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

Kat-Nr. : 16183-1-AP

Synonyme

F11R, JAM-A/Junctional Adhesion Molecule A, JAM A, JAM 1, JAM

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  WB analysis of mouse liver using 16183-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16183-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 16183-1-AP

  IP result of anti-JAM-A/Junctional Adhesion Molecule A (IP:16183-1-AP, 4ug; Detection:16183-1-AP 1:300) with mouse liver tissue lysate 3000ug.

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  IHC staining of human breast cancer using 16183-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 16183-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16183-1-AP (JAM-A/Junctional Adhesion Molecule A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, human peripheral blood platelets
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below

Produktinformation

16183-1-AP bindet in WB, IHC, IP, ELISA JAM-A/CD321 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: F11 receptor
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC001533
• Gene symbol: F11R
• Gene ID (NCBI): 50848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and a ligand for the integrin LFA1.

Publikationen

Species	Application	Title
human	WB,IHC	Front Oncol; The Long Non-Coding RNA HOXC-AS3 Promotes Glioma Progression by Sponging miR-216 to Regulate F11R Expression.; Authors - Yongshuai Li
human	WB	Clin Exp Pharmacol Physiol; Rabeprazole destroyed gastric epithelial barrier function through FOXF1/STAT3-mediated ZO-1 expression; Authors - Fangying Yang
rat	WB	J Chem Neuroanat; Matrine treatment induced an A2 astrocyte phenotype and protected the blood-brain barrier in CNS autoimmunity.; Authors - Yilei Jing
human	WB	Biomedicines; Identification and Validation of JAM-A as a Novel Prognostic and Immune Factor in Human Tumors; Authors - Tianyi Ren
mouse	WB	Int J Biol Macromol; Epicatechin and β-glucan from whole highland barley grain ameliorates hyperlipidemia associated with attenuating intestinal barrier dysfunction and modulating gut microbiota in high-fat-diet-fed mice; Authors - Zehua Liu