F11R Polyklonaler Antikörper

F11R Polyklonal Antikörper für FC, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26941-1-AP

Synonyme

CD321, F11 receptor, F11R, JAM, JAM 1, JAM A, JAM1, JAMA, JAMA1, JCAM, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, PAM 1, Platelet adhesion molecule 1, Platelet F11 receptor

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Galerie der Validierungsdaten

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  WB analysis using 26941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 26941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human peripheral blood platelets using 26941-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 26941-1-AP (F11R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26941-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26941-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 26941-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 26941-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 26941-1-AP (F11R antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of U-87 MG using 26941-1-AP

  1X10^6 U-87 MG cells were surface stained with 0.8 ug Anti-Human F11R (26941-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were not fixed .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: U-87 MG-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26941-1-AP bindet in WB, IHC, FC, ELISA F11R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: F11R fusion protein Ag25641
• Vollständiger Name: F11 receptor
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC001533
• Gene symbol: F11R
• Gene ID (NCBI): 50848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The F11 receptor (F11R) was first identified in human platelets as a 32/35 kDa protein duplex that serves as the receptor for a functional antibody that activates platelets. It seems to play a role in epithelial tight junction formation. It was also reported to function as a receptor for reovirus and ligand for the integrin LFA1.