Factor XII Polyklonaler Antikörper

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• KD/KO Validated

Factor XII Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12551-1-AP

Synonyme

Coagulation factor XII, F12, Factor XII, HAE3, HAEX, HAF, Hageman factor

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Galerie der Validierungsdaten

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  WB analysis using 12551-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12551-1-AP (Factor XII antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 12551-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12551-1-AP (Factor XII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 12551-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12551-1-AP (Factor XII antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12551-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12551-1-AP (Factor XII antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12551-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12551-1-AP (Factor XII antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12551-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12551-1-AP (Factor XII antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

12551-1-AP bindet in WB, IHC, IF, ELISA Factor XII und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Factor XII fusion protein Ag3401
• Vollständiger Name: coagulation factor XII (Hageman factor)
• Berechnetes Molekulargewicht: 615 aa, 68 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC012390
• Gene symbol: F12
• Gene ID (NCBI): 2161
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Factor XII (F XII, Hageman factor) is a 80 kDa, single chain glycoprotein that circulates in blood as an inactive zymogen. F XII plays an important role in blood coagulation, fibrinolysis, and kinin generation. Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of a 52 kDa NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a 28 kDa COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Blood coagulation factor XII drives adaptive immunity during neuroinflammation via CD87-mediated modulation of dendritic cells.; Authors - Kerstin Göbel
human	IHC	Thyroid; Clinical and Molecular Characterizations of Papillary Thyroid Cancer in Children and Young Adults: a Multicenter Retrospective Study.; Authors - Kai Guo
mouse	WB,IHC	Int J Mol Med; FXII regulates the formation of deep vein thrombosis via the PI3K/AKT signaling pathway in mice.; Authors - Yan Meng ; KD Validated