F2 Polyklonaler Antikörper

F2 Polyklonal Antikörper für IF, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 24295-1-AP

Synonyme

Coagulation factor II, F2, Prothrombin, PT

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 24295-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 24295-1-AP (F2 Antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 24295-1-AP

  IP Result of anti-F2 (IP:24295-1-AP, 4ug; Detection:24295-1-AP 1:10000) with human plasma lysate 4000ug.

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  IF Staining of HUVEC using 24295-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HUVEC cells using 24295-1-AP (F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche IP: humanes Plasmagewebe
• Erfolgreiche Detektion in IF: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 3 publications below

Produktinformation

24295-1-AP bindet in WB, IP, IF, ELISA F2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Plasmodium falciparum
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: F2 fusion protein Ag18385
• Vollständiger Name: coagulation factor II (prothrombin)
• Berechnetes Molekulargewicht: 622 aa, 70 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC051332
• Gene symbol: F2
• Gene ID (NCBI): 2147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Factor II, or prothrombin, is one of the components of this chain of plasma proteins involved in blood coagulation. Thrombin is essential in the processes of hemostasis and thrombosis.

Publikationen

Species	Application	Title
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
human	WB	Sci Rep; Molecular Camouflage of Plasmodium falciparum Merozoites by Binding of Host Vitronectin to P47 Fragment of SERA5.; Authors - Takahiro Tougan
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
Plasmodium falciparum	WB,IF	Malar J; The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.; Authors - Takahiro Tougan
human	IF	PLoS Pathog; Thrombin cleavage of the hepatitis E virus polyprotein at multiple conserved locations is required for genome replication; Authors - Danielle M Pierce

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Elke (Verified Customer) (02-23-2023)

it can work on the western blot

• Applications: Western Blot