Coagulation Factor II Monoklonaler Antikörper

Coagulation Factor II Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1E12A8

Kat-Nr. : 66509-1-Ig

Synonyme

F2, Prothrombin, 1E12A8, Activation peptide fragment 1, Activation peptide fragment 2

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  WB analysis of human plasma using 66509-1-Ig

  Untreated and PNGase F-treated lysates of human plasma were subjected to SDS PAGE followed by western blot with 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

• 

  WB analysis of human plasma using 66509-1-Ig

  human plasma was subjected to SDS PAGE followed by western blot with 66509-1-Ig (Coagulation Factor II antibody) at various dilutions incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:150 (under 10x lens).

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  IHC staining of human liver using 66509-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66509-1-Ig (Coagulation Factor II antibody) at dilution of 1:150 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

66509-1-Ig bindet in WB, IHC, ELISA Coagulation Factor II und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Hausschwein
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Coagulation Factor II fusion protein Ag18385
• Vollständiger Name: coagulation factor II (prothrombin)
• Berechnetes Molekulargewicht: 622 aa, 70 kDa
• Beobachtetes Molekulargewicht: 70-80 kDa
• GenBank-Zugangsnummer: BC051332
• Gene symbol: F2
• Gene ID (NCBI): 2147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Coagulation Factor II, also known as prothrombin or factor II, is one of the components of this chain of plasma proteins involved in blood coagulation. Prothrombin is the precursor of thrombin, which is essential in the processes of hemostasis and thrombosis.

Publikationen

Species	Application	Title
pig	WB	Vet Res; TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus; Authors - Xuejiao Zhu