PAR1/Thrombin Receptor Polyklonaler Antikörper

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• KD/KO Validated

PAR1/Thrombin Receptor Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 26366-1-AP

Synonyme

F2R, CF2R, Proteinase-activated receptor 1, PAR-1, PAR1

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• 

  WB analysis using 26366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26366-1-AP (PAR1/Thrombin Receptor antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 26366-1-AP

  WB result of F2R antibody (26366-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAR1/Thrombin Receptor transfected K-562 cells.

• 

  IHC staining of human liver using 26366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 26366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26366-1-AP (PAR1/Thrombin Receptor Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

26366-1-AP bindet in WB, IHC, IF, IP, ELISA PAR1/Thrombin Receptor und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Affe, hamster, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PAR1/Thrombin Receptor fusion protein Ag24378
• Vollständiger Name: coagulation factor II (thrombin) receptor
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC051909
• Gene symbol: F2R
• Gene ID (NCBI): 2149
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protease-activated receptor-1 (PAR1) is a G protein coupled receptor.PAR1 has a greater affinity for thrombin and mediates rapid Ca2+ mobilization and platelet activation. PAR1 has a calculated molecular weight of 47 kDa, but can be detected as 70 kDa after posttranslational modification.(PMID: 26822533)

Publikationen

Species	Application	Title
human,mouse	WB,IHC,IP	Breast Cancer Res Treat; Exosomal MMP-1 transfers metastasis potential in triple-negative breast cancer through PAR1-mediated EMT.; Authors - Yihui Zhu
hamster	WB	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu
human,monkey	WB,IHC,IF	Genes Immun; A Golgi apparatus-related signature predicts the immune microenvironment and prognosis of gastric cancer; Authors - Changlei Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sammy (Verified Customer) (02-15-2024)

Several unspecific bands detected by WB one of which was the correct size. IF staining worked well but caveated by potential lack of specificity.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1 in 1000 WB 1 in 200 IF
• Cell Tissue Type: HUVEC lysate