F2RL3 Polyklonaler Antikörper

F2RL3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 25306-1-AP

Synonyme

Proteinase-activated receptor 4, Proteinase activated receptor 4, PAR-4, PAR4, PAR 4

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  WB analysis using 25306-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 25306-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 25306-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 25306-1-AP (F2RL3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse lung using 25306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 25306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25306-1-AP (F2RL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe, Maus-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Mauslungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

25306-1-AP bindet in WB, IHC, ELISA F2RL3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: F2RL3 fusion protein Ag20801
• Vollständiger Name: coagulation factor II (thrombin) receptor-like 3
• Berechnetes Molekulargewicht: 385 aa, 41 kDa
• Beobachtetes Molekulargewicht: 41 kDa
• GenBank-Zugangsnummer: BC074782
• Gene symbol: F2RL3
• Gene ID (NCBI): 9002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Coagulation factor II receptor-like 3 (F2RL3) encodes a member of the protease-activated receptor subfamily, also known as protease-activated receptor 4 (PAR4), which takes partin platelet activation, intimal hyperplasia and inflammation (PMID:34284820). An absence of PAR4 in mouse models results in impaired hemostasis and a protection against pulmonary embolism, and a small number of missense coding variants in F2RL3 that alter platelet aggregation and function have been described(PMID:35012325). The calculated molecular weight and observed molecular weight of F2RL3 are both 41 kDa.

Publikationen

Species	Application	Title
human	WB	Oxid Med Cell Longev; Combination of Colchicine and Ticagrelor Inhibits Carrageenan-Induced Thrombi in Mice.; Authors - BuChun Zhang
mouse	WB	Front Oncol; Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity.; Authors - Nathalia Araujo
mouse	WB	Neurosci Bull; Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.; Authors - Jianing Luo
mouse	WB	Cells; The Tumor Suppressor Par-4 Regulates Adipogenesis by Transcriptional Repression of PPARγ; Authors - James Sledziona