F4/80 Rekombinanter Antikörper

F4/80 Rekombinant Antikörper für IHC, IF/ICC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF/ICC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2P18

Kat-Nr. : 81668-1-RR

Synonyme

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

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  IHC staining of mouse spleen using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 81668-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 81668-1-RR (F4/80 antibody) at dilution of 1:3000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 81668-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse spleen using 81668-1-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse spleen using 81668-1-RR

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of RAW 264.7 using 81668-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody (81668-1-RR, Clone: 2P18 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81668-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81668-1-RR against Mouse F4/80 were performed. The affinity constant is 14.4 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe, RAW 264.7-Zellen
• Erfolgreiche Detektion in IF-Fro: Mausmilzgewebe
• Erfolgreiche Detektion in IF/ICC: RAW 264.7-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:3000
• Immunfluoreszenz (IF)-P: IF-P : 1:750-1:3000
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 2 publications below

Produktinformation

81668-1-RR bindet in IHC, IF/ICC, IF-P, IF-Fro, ELISA F4/80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: F4/80 fusion protein Ag28252
• Vollständiger Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Berechnetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: NM_010130
• Gene symbol: F4/80
• Gene ID (NCBI): 13733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.

Publikationen

Species	Application	Title
mouse	IF	bioRxiv; Integrin alpha1 beta1 promotes interstitial fibrosis in a mouse model of polycystic kidney disease; Authors - C Grenier
		Genes Immun; Annexin A1 regulates inflammatory-immune response and reduces pancreatic and extra- pancreatic injury during severe acute pancreatitis; Authors - Shizhao Lin
mouse	IF	Cell Rep; The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring; Authors - Chunxiang Shen