F4/80 Rekombinanter Antikörper

F4/80 Rekombinant Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

241959B6

Kat-Nr. : 84538-1-RR

Synonyme

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

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  IHC staining of mouse spleen using 84538-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with .

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  IHC staining of mouse spleen using 84538-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with .

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  IHC staining of mouse liver using 84538-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 84538-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84538-1-RR (F4/80 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 84538-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using F4/80 antibody (84538-1-RR, Clone: 241959B6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 84538-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using F4/80 antibody (84538-1-RR, Clone: 241959B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84538-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84538-1-RR against Mouse F4/80 were performed. The affinity constant is 1.22 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausmilzgewebe, Mauslebergewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84538-1-RR bindet in IHC, IF-P, ELISA F4/80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Berechnetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: NM_010130.4
• Gene symbol: F4/80
• Gene ID (NCBI): 13733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mouse F4/80, also named as EMR1 and Gpf480, is a 160kd cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.