F4/80 Rekombinanter Antikörper

F4/80 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241959G4

Kat-Nr. : 98236-1-PBS

Synonyme

241959G4, Adgre1, Adhesion G protein-coupled receptor E1, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1

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  FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit RecAb (98236-1-RR, Clone: 241959G4) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Mouse CD11b (M1/70) (APC-65055, Clone: M1/70). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Balb/c mouse peritoneal macrophages using 98236-1-RR (same clone as 98236-1-PBS)

  1x10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse F4/80 Rabbit RecAb (98236-1-RR, Clone: 241959G4) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98236-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98236-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98236-1-RR against Mouse F4/80 were performed. The affinity constant is 4.50 nM.

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Geprüfte Anwendungen

Produktinformation

98236-1-PBS bindet in FC F4/80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: F4/80 fusion protein Eg0788
• Vollständiger Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Berechnetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: NM_010130.4
• Gene symbol: F4/80
• Gene ID (NCBI): 13733
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.