F4/80 Rekombinanter Antikörper

F4/80 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

241959G4

Kat-Nr. : CL750-98236

Synonyme

Emr1, EGF-like module-containing mucin-like hormone receptor-like 1, EGF-like module receptor 1, Adhesion G protein-coupled receptor E1, Adgre1

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  FC experiment of mouse peritoneal macrophages using CL750-98236

  1x10^6 mouse peritoneal macrophages were surface stained with FITC Plus Anti-Mouse CD11b, and 0.25 ug CoraLite® Plus 750 Anti-Mouse F4/80 Rabbit RecAb (CL750-98236, Clone: 241959G4) or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of mouse peritoneal macrophages using CL750-98236

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug CoraLite® Plus 750 Anti-Mouse F4/80 Rabbit RecAb (CL750-98236, Clone: 241959G4) (red) or 0.25 ug CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: peritoneale Makrophagen der Maus

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-98236 bindet in FC F4/80 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: EGF-like module containing, mucin-like, hormone receptor-like sequence 1
• Berechnetes Molekulargewicht: 102 kDa
• GenBank-Zugangsnummer: NM_010130.4
• Gene symbol: F4/80
• Gene ID (NCBI): 13733
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Mouse F4/80, also named as EMR1 and Gpf480, is a 160-kDa cell surface glycoprotein which is a member of the EGF TM7 family. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. The function of F4/80 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.