Factor VII Polyklonaler Antikörper

Factor VII Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 23058-1-AP

Synonyme

F7, Coagulation factor VII, EC:3.4.21.21, Eptacog alfa, Proconvertin

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  WB analysis using 23058-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 23058-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 23058-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 23058-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 23058-1-AP (F7 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HUVEC using 23058-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using F7 antibody (23058-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Plazenta-Gewebe
• Erfolgreiche Detektion in IF/ICC: HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

23058-1-AP bindet in WB, IF/ICC, ELISA Factor VII und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Factor VII fusion protein Ag19324
• Vollständiger Name: coagulation factor VII (serum prothrombin conversion accelerator)
• Berechnetes Molekulargewicht: 466 aa, 52 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC130468
• Gene symbol: F7
• Gene ID (NCBI): 2155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

F7(Coagulation factor VII) is also named as serum prothrombin conversion accelerator(SPCA). It is the key triggering enzyme of the extrinsic coagulation pathway. Hepatocytes are the predominant source of F7 production under normal physiologic conditions, although F7 is also expressed by monocytes/macrophages in inflammation and atherosclerosis(PMID:17018600). This protein has 2 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
human	WB	Sci Rep; C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1.; Authors - Anika Alberts
mouse	WB	Drug Des Devel Ther; Guanxinning Tablet Alleviates Post-Ischemic Stroke Injury Via Regulating Complement and Coagulation Cascades Pathway and Inflammatory Network Mobilization; Authors - Yule Wang
pig	WB	Vet Res; TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus; Authors - Xuejiao Zhu ; KD Validated