F8A2 Polyklonaler Antikörper

F8A2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32696-1-AP

Synonyme

40-kDa huntingtin-associated protein, Coagulation Factor VIII Associated 2, CpG island protein, F8A1, F8A3

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  WB analysis using 32696-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32696-1-AP (F8A2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 32696-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using F8A2 antibody (32696-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SKOV-3-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32696-1-AP bindet in WB, IF/ICC, ELISA F8A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: F8A2 fusion protein Ag38529
• Vollständiger Name: coagulation factor VIII-associated (intronic transcript) 2
• Berechnetes Molekulargewicht: 39kDa，371aa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: NM_012151.3
• Gene symbol: F8A2
• Gene ID (NCBI): 474383
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

F8A2 (Coagulation Factor VIII Associated 2) is a protein associated with coagulation factor VIII, which is widely expressed in a variety of cell types, localized mainly in the cytoplasm and nucleus, and also co-localized with Huntington's protein (HTT). Although its specific function is not fully defined, F8A2 may play an important role in intracellular and vesicular transport. In addition, F8A2 co-localizes with ubiquitin in neuronal cells and may be involved in the pathological process of neurodegenerative diseases.