Factor IX/PTC Polyklonaler Antikörper

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• KD/KO Validated

Factor IX/PTC Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 21481-1-AP

Synonyme

F9, Factor IX, EC:3.4.21.22, Coagulation factor IXa light chain, Coagulation factor IXa heavy chain

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  WB analysis of HepG2 using 21481-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 21481-1-AP (F9 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

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  IHC staining of human liver cancer using 21481-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21481-1-AP (F9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 21481-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21481-1-AP (F9 Antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit Tunicamycin behandelte HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

21481-1-AP bindet in WB, IHC, IF, ELISA Factor IX/PTC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Factor IX/PTC fusion protein Ag15723
• Vollständiger Name: coagulation factor IX
• Berechnetes Molekulargewicht: 461 aa, 52 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC109215
• Gene symbol: F9
• Gene ID (NCBI): 2158
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The F9 gene encodes coagulation factor IX, which circulates as an inactive zymogen until proteolytic release of its activation peptide allows it to assume the conformation of an active serine protease. Its role in the blood coagulation cascade is to activate factor X (F10) through interactions with calcium, membrane phospholipids, and factor VIII (F8). Factor IX and factor X both consist of 2 polypeptide chains referred to as the L (light) and H (heavy) chains. The H chain bears a structural resemblance to the polypeptide chain of the pancreatic serine protease trypsin (PRSS1). The L chain is covalently linked to the H chain by a single disulfide bond.

Publikationen

Species	Application	Title
mouse	WB	EMBO Mol Med; CRISPR/Cas9-mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse.; Authors - Yuting Guan
human	WB	Front Neurosci; Serum Exosomal Proteins F9 and TSP-1 as Potential Diagnostic Biomarkers for Newly Diagnosed Epilepsy.; Authors - Zijun Lin
human	IF	Cell Death Dis; Genome wide CRISPR/Cas9 screen identifies the coagulation factor IX (F9) as a regulator of senescence.; Authors - Paula Carpintero-Fernández ; KD Validated
pig	WB	Vet Res; TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus; Authors - Xuejiao Zhu ; KD Validated