FAAH Polyklonaler Antikörper

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• KD/KO Validated

FAAH Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 17909-1-AP

Synonyme

Anandamide amidohydrolase 1, FAAH, FAAH 1, FAAH1, fatty acid amide hydrolase, Fatty acid amide hydrolase 1, Oleamide hydrolase 1

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Galerie der Validierungsdaten

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  WB analysis using 17909-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17909-1-AP

  WB result of FAAH antibody (17909-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAAH transfected A431 cells.

• 

  WB analysis of rat testis using 17909-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 17909-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17909-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17909-1-AP (FAAH Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 17909-1-AP

  IP result of anti-FAAH (IP:17909-1-AP, 4ug; Detection:17909-1-AP 1:500) with mouse liver tissue lysate 6400 ug.

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  IHC staining of mouse testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17909-1-AP (FAAH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17909-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17909-1-AP (FAAH Antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, A431-Zellen, Rattenhodengewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Hodengewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:300

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IP: See 1 publications below

Produktinformation

17909-1-AP bindet in WB, IP, IHC, ELISA FAAH und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAAH fusion protein Ag12304
• Vollständiger Name: fatty acid amide hydrolase
• Berechnetes Molekulargewicht: 579 aa, 63 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC093632
• Gene symbol: FAAH
• Gene ID (NCBI): 2166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Publikationen

Species	Application	Title
mouse	WB	Elife; p21-activated kinase 1 restricts tonic endocannabinoid signaling in the hippocampus.; Authors - Shuting Xia
rat	WB	J Proteome Res; Joint Synovial Fluid Metabolomics Method to Decipher the Metabolic Mechanisms of Adjuvant Arthritis and Geniposide Intervention.; Authors - Xiang Zhan
mouse	WB	Neuroscience; The Endocannabinoid System Contributes to Memory Deficits Induced by Rapid-eye-movement Sleep Deprivation in Adolescent Mice.; Authors - Jinming Zhang
human,mouse	WB,IP	Cell Death Differ; FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system; Authors - Yangyang Zhu ; KO Validated
human	WB	Cell Death Dis; Combination treatment with FAAH inhibitors/URB597 and ferroptosis inducers significantly decreases the growth and metastasis of renal cell carcinoma cells via the PI3K-AKT signaling pathway; Authors - Junfeng Hao