FAAH Monoklonaler Antikörper

FAAH Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3C5B5

Kat-Nr. : 67607-1-Ig

Synonyme

3C5B5, EC:3.1.1.-, EC:3.5.1.99, FAAH1, Fatty acid ester hydrolase

---

• 

  WB analysis using 67607-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of JAR using 67607-1-Ig

  JAR cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of LNCaP using 67607-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 410x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67607-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of LNCaP using 67607-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using FAAH antibody (67607-1-Ig, Clone: 3C5B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, JAR-Zellen, LNCaP-Zellen, Hausschwein-Lebergewebe, Kaninchenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: LNCaP-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

67607-1-Ig bindet in WB, IHC, IF/ICC, ELISA FAAH und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FAAH fusion protein Ag12720
• Vollständiger Name: fatty acid amide hydrolase
• Berechnetes Molekulargewicht: 579 aa, 63 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC093632
• Gene symbol: FAAH
• Gene ID (NCBI): 2166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell Neurosci; Cannabinoid receptor 2 agonist AM1241 alleviates epileptic seizures and epilepsy-associated depression via inhibiting neuroinflammation in a pilocarpine-induced chronic epilepsy mouse model; Authors - Yiying Cai