FAAH Monoklonaler Antikörper

FAAH Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3C5B5

Kat-Nr. : 67607-1-PBS

Synonyme

3C5B5, EC:3.1.1.-, EC:3.5.1.99, FAAH1, Fatty acid ester hydrolase

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  WB analysis using 67607-1-Ig (same clone as 67607-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 67607-1-Ig (same clone as 67607-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67607-1-Ig (FAAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 410x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67607-1-Ig (FAAH antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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  IF Staining of LNCaP using 67607-1-Ig (same clone as 67607-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed LNCaP cells using FAAH antibody (67607-1-Ig, Clone: 3C5B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67607-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67607-1-PBS bindet in WB, IHC, IF/ICC, ELISA FAAH und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FAAH fusion protein Ag12720
• Vollständiger Name: fatty acid amide hydrolase
• Berechnetes Molekulargewicht: 579 aa, 63 kDa
• Beobachtetes Molekulargewicht: 55-65 kDa
• GenBank-Zugangsnummer: BC093632
• Gene symbol: FAAH
• Gene ID (NCBI): 2166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid amide hydrolase (FAAH) is a membrane-bound homodimeric enzyme that in vivo controls content and biological activity of N-arachidonoylethanolamine (AEA) and other relevant bioactive lipids termed endocannabinoids. (PMID: 32041998) This antibody is specific for FAAH protein.