FABP1 Monoklonaler Antikörper

FABP1 Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B6C8

Kat-Nr. : 68227-1-PBS

Synonyme

liver FABP, 1B6C8, FABPL, Fatty acid binding protein 1, Fatty acid-binding protein 1

---

• 

  WB analysis using 68227-1-Ig (same clone as 68227-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig liver using 68227-1-Ig (same clone as 68227-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68227-1-Ig (FABP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68227-1-Ig (FABP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68227-1-Ig (FABP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse liver using 68227-1-Ig (same clone as 68227-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using FABP1 antibody (68227-1-Ig, Clone: 1B6C8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 68227-1-Ig (same clone as 68227-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug FABP1 Monoclonal antibody (68227-1-Ig, Clone:1B6C8) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68227-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

68227-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA FABP1 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FABP1 fusion protein Ag32836
• Vollständiger Name: fatty acid binding protein 1, liver
• Berechnetes Molekulargewicht: 127 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC032801
• Gene symbol: FABP1
• Gene ID (NCBI): 2168
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP1, liver fatty acid-binding protein, is abundant in cytoplasm that regulates lipid transport and metabolism. It plays a role in lipoprotein-mediated cholesterol uptake in hepatocytes (PMID:25732850). FABP1 is mainly expressed in hepatocytes, enterocytes and to a lesser degree in renal tubular cells, associated with liver injury (PMID: 15653098). Levels of FABP1 was elevated in patients with hepatocyte injury secondary to alcohol or drug toxicity (PMID: 14563446).