FABP2 Monoklonaler Antikörper

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• KD/KO Validated

FABP2 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2D11G6

Kat-Nr. : 67691-1-Ig

Synonyme

2D11G6, FABPI, Fatty acid binding protein 2, Fatty acid-binding protein 2, Fatty acid-binding protein, intestinal

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  WB analysis using 67691-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 67691-1-Ig

  WB result of FABP2 antibody (67691-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP2 transfected COLO 320 cells.

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  WB analysis using 67691-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67691-1-Ig (FABP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 67691-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67691-1-Ig (FABP2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 67691-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using FABP2 antibody (67691-1-Ig, Clone: 2D11G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Dünndarmgewebe, COLO 320-Zellen, humanes Jejunum-Gewebe
• Erfolgreiche Detektion in IHC: Maus-Dünndarmgewebe, humanes Dünndarmgewebe, Maus-Kolongewebe, Ratten-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Kolongewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:50000
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 1 publications below

Produktinformation

67691-1-Ig bindet in WB, IHC, IF-P, ELISA FABP2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FABP2 fusion protein Ag17620
• Vollständiger Name: fatty acid binding protein 2, intestinal
• Berechnetes Molekulargewicht: 132 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC069617
• Gene symbol: FABP2
• Gene ID (NCBI): 2169
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP2, also known as the intestinal fatty acid binding protein (I-FABP), is expressed in the absorptive intestinal villus cells. It is mainly involved in intracellular transport and intestinal absorption of lipids. FABP2 has been considered a marker of mucosal injury and ischemia and serum I-FABP level is used as a tissue damage indicator. In addition, it is a marker of differentiated intestinal epithelial cells.

Publikationen

Species	Application	Title
mouse	WB	Int J Mol Sci; Anti-Proteolytic Peptide R7I Protects the Intestinal Barrier and Alleviates Fatty Acid Malabsorption in Salmonella typhimurium-Infected Mice; Authors - Yunzhe Su
mouse	IF	Gut Microbes; Infant feces-derived Lactobacillus gasseri FWJL-4 mitigates experimental necrotizing enterocolitis via acetate production; Authors - Li-Long Pan
	WB	Cell Biochem Funct; Neutrophil Depletion Reduced the Relative Abundance of Unsaturated Long-Chain Fatty Acid Synthesis Microbiota and Intestinal Lipid Absorption; Authors - Xingyu Lu