FABP4 Polyklonaler Antikörper

FABP4 Polyklonal Antikörper für IF, IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

12

Kat-Nr. : 15872-1-AP

Synonyme

A FABP, AFABP, ALBP, aP2, FABP4, Fatty acid binding protein 4

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Galerie der Validierungsdaten

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  WB analysis of rat skeletal muscle using 15872-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15872-1-AP (FABP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15872-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15872-1-AP (FABP4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervix using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 15872-1-AP (FABP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervix using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervix using 15872-1-AP (FABP4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 15872-1-AP (FABP4 Antibody) at dilution of 1:50.

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  IHC staining of human skin using 15872-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin slide using 15872-1-AP (FABP4 Antibody) at dilution of 1:50.

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  IF Staining of mouse adipose using 15872-1-AP

  IF result of anti-FABP4 (15872-1-AP, 1:500) with PFA fixed mouse adipose tissue by Dr. Daniel Kopinke.

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  IF Staining of HUVEC using 15872-1-AP

  Immunofluorescent analysis of HUVEC cells using 15872-1-AP (FABP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Ratten-Skelettmuskelgewebe, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Zervixgewebe, humanes Hautgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: mouse adipose, HUVEC-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

15872-1-AP bindet in WB, IHC, IF, ELISA FABP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FABP4 fusion protein Ag8631
• Vollständiger Name: fatty acid binding protein 4, adipocyte
• Berechnetes Molekulargewicht: 132 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC003672
• Gene symbol: FABP4
• Gene ID (NCBI): 2167
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid binding protein (FABP) 4 is a member of the FABP family which abundantly expressed, fatty acid carrier proteins. FABPs are capable of binding a variety of hydrophobic molecules such as long-chain fatty acids and are important for their uptake and intracellular trafficking. It was first identified as an adipocyte-specific protein, important for the maintenance of lipid and glucose metabolism. It is also detected in macrophages, where it participates in regulating inflammation and cholesterol trafficking via NFκB and PPAR. In more recent studies, FABP4 has been found in a variety of endothelial cells, where it has been identified as a target of VEGF and a regulator of cell proliferation and possibly angiogenesis. Pathologically, FABP4 has been associated with the development of metabolic syndrome, diabetes and cancer and vulnerability of atherosclerotic plaques. FABP4 has been identified as a novel prognostic factor for both adverse cardiovascular events and breast cancer.

Publikationen

Species	Application	Title
mouse	IF	Cell; Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging.; Authors - Marion Claudia Salzer
human	IHC	Eur Heart J; Adipocyte fatty acid binding protein in atherosclerotic plaques is associated with local vulnerability and is predictive for the occurrence of adverse cardiovascular events.; Authors - Peeters Wouter W
mouse	WB,IF	Autophagy; Autophagy loss impedes cancer-associated fibroblast activation via downregulating proline biosynthesis.; Authors - Jingru Bai
mouse	IF	PLoS Biol; Single-cell mapping reveals new markers and functions of lymphatic endothelial cells in lymph nodes.; Authors - Noriki Fujimoto
human	WB	Int J Obes (Lond); miR-20a regulates adipocyte differentiation by targeting lysine-specific demethylase 6b and transforming growth factor-β signaling.; Authors - J Zhou
		Am J Physiol Cell Physiol; Human Adipocytes from the Subcutaneous Superficial Layer have Greater Adipogenic Potential and Lower PPAR-γ DNA Methylation Levels than Deep Layer Adipocytes.; Authors - Kentaro Kosaka

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