FABP5 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FABP5 Polyklonal Antikörper für FC, IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

34

Rezensionen (1)

Kat-Nr. : 12348-1-AP

Synonyme

E FABP, EFABP, FABP5, Fatty acid binding protein 5, PA FABP, PAFABP

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Galerie der Validierungsdaten

• 

  WB analysis of A375 using 12348-1-AP

  WB result of FABP5 antibody (12348-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells.

• 

  WB analysis using 12348-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 12348-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12348-1-AP (FABP5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U2OS using 12348-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 12348-1-AP (FABP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 12348-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.20 ug/test Anti-Human FABP5 (12348-1-AP) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Fixed with 90% MeOH.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A431-Zellen, HeLa-Zellen, HepG2-Zellen, Maushautgewebe
• Erfolgreiche Detektion in IF: U2OS-Zellen
• Erfolgreiche Detektion in FC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 29 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• CoIP: See 2 publications below

Produktinformation

12348-1-AP bindet in WB, IHC, IF, FC, CoIP, ELISA FABP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FABP5 fusion protein Ag3005
• Vollständiger Name: fatty acid binding protein 5 (psoriasis-associated)
• Berechnetes Molekulargewicht: 135 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC019385
• Gene symbol: FABP5
• Gene ID (NCBI): 2171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Publikationen

Species	Application	Title
human,mouse	WB, CoIP	Nat Commun; Oxidative stress-induced FABP5 S-glutathionylation protects against acute lung injury by suppressing inflammation in macrophages.; Authors - Yuxian Guo
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
human	IF	Mol Ther; Single-Cell Transcriptome Analysis Reveals Intratumoral Heterogeneity in ccRCC, which Results in Different Clinical Outcomes.; Authors - Junyi Hu
human	WB	EMBO Rep; Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.; Authors - Yang Zhang
mouse	IF	Antioxid Redox Signal; Dual inhibition of PI3K/Akt and mTOR by the Dietary Antioxidant Delphinidin Ameliorates Psoriatic Features In-vitro and in an Imiquimod-induced Psoriasis-like Disease in Mice.; Authors - Jean Christopher Chamcheu
human	WB	Cell Death Dis; The natural compound GL22, isolated from Ganoderma mushrooms, suppresses tumor growth by altering lipid metabolism and triggering cell death.; Authors - Ge Liu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kenzo (Verified Customer) (01-09-2023)

Robust immunoreactivity detected.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse nerve

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