FABP5 Monoklonaler Antikörper

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• KD/KO Validated

FABP5 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(5)

CloneNo.

1C6E12

Kat-Nr. : 66299-1-Ig

Synonyme

1C6E12, EFABP, Epidermal-type fatty acid-binding protein, Fatty acid binding protein 5, Fatty acid-binding protein 5

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  WB analysis using 66299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis of A375 using 66299-1-Ig

  WB result of FABP5 antibody (66299-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FABP5 transfected A375 cells.

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  WB analysis of Hepa1-6 using 66299-1-Ig

  WB result of FABP5 antibody (66299-1-Ig; 1:1000; room temperature for 1.5 hours) with wild-type and FABP5 knockout Hepa1-6 cells.

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  WB analysis of A375 using 66299-1-Ig

  A375 cells were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66299-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 66299-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66299-1-Ig (FABP5 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66299-1-Ig (FABP5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 66299-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66299-1-Ig (FABP5 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 66299-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FABP5 antibody (66299-1-Ig, Clone: 1C6E12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, A549-Zellen, fetales humanes Hirngewebe, HEK-293-Zellen, HeLa-Zellen, Hepa1-6-Zellen, Maushirngewebe, Rattenhirngewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Prostatakarzinomgewebe, braunes Mausfettgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:200-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Produktinformation

66299-1-Ig bindet in WB, IHC, IF/ICC, ELISA FABP5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FABP5 fusion protein Ag3005
• Vollständiger Name: fatty acid binding protein 5 (psoriasis-associated)
• Berechnetes Molekulargewicht: 135 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC019385
• Gene symbol: FABP5
• Gene ID (NCBI): 2171
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP5, also named as PA-FABP and E-FABP, belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. It is high specificity for fatty acids. FABP5 is highest affinity for C18 chain length. It may be involved in keratinocyte differentiation. FABP5 is a fatty acid-binding protein and is expressed in epidermis and endothelial cells of the microvasculature of different organs. FABP5 has also been identified as a tumor-associated antigen, which is highly expressed in various cancers. FABP5 was detected in the sera of HNSCC patients with early stage cancer. Antibodies specific for FABP5 were significantly increased in a substantial amount in patients, suggesting that FABP5 may be a potential diagnostic biomarker for HNSCC. FABP5 may serve as a biomarker for HNSCC.(PMID:19602232)

Publikationen

Species	Application	Title
human	WB	Front Oncol; Multi-Omics Analysis of Fatty Acid Metabolism in Thyroid Carcinoma.; Authors - Jinghui Lu
human	WB,IHC	Carcinogenesis; Serum fatty acid-binding protein 5 is a significant factor in hepatocellular carcinoma progression independent of tissue expression level.; Authors - Masafumi Ohira ; KO Validated
human	IHC	Med Mol Morphol; Expression of fatty-acid-binding protein 5 in intrahepatic and extrahepatic cholangiocarcinoma: the possibility of different energy metabolisms in anatomical location.; Authors - Risa Nakagawa
mouse	IF	J Allergy Clin Immunol; Melatonin treatment increases skin microbiota-derived propionic acid to alleviate atopic dermatitis; Authors - Lan Yang
human	IHC,IF	Free Radic Biol Med; Oxidative stress-induced decreased expression of FABP5 leads to mitochondrial damage and survival disorder of decidual stromal cells in women with recurrent spontaneous abortion; Authors - Dong Li