FABP7 Polyklonaler Antikörper

FABP7 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 51010-1-AP

Synonyme

B FABP, BLBP, Brain lipid binding protein, FABP7, FABPB, Fatty acid binding protein 7, MRG

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Galerie der Validierungsdaten

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  WB analysis of human brain using 51010-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 51010-1-AP (FABP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 51010-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51010-1-AP (FABP7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human malignant melanoma using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human malignant melanoma using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 51010-1-AP (FABP7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 51010-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 51010-1-AP (FABP7 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of rat brain using 51010-1-AP

  Immunofluorescent analysis of (-20°C Acetone) fixed rat brain tissue using FABP7 antibody (51010-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Hirngewebe, Maus-Fettgewebe
• Erfolgreiche Detektion in IHC: humanes malignes Melanomgewebe, humanes Gliomgewebe, humanes Hirngewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

51010-1-AP bindet in WB, IHC, IF, ELISA FABP7 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: fatty acid binding protein 7, brain
• Berechnetes Molekulargewicht: 132aa,15 kDa; 166aa,19 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC012299
• Gene symbol: FABP7
• Gene ID (NCBI): 2173
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FABP7 (brain fatty acid-binding protein; BLBP) is a member of the FABP family of lipid chaperones involved in the uptake and intracellular trafficking of fatty acids. FABP7 is expressed in radial glial cells during brain development. FABP7 is up-regulated in brain tumor tissue; its expression has been associated with decreased survival times and tumor progression in patients with astrocytoma, melanoma, basal-type breast cancer, and renal cell carcinoma. This antibody is useful to label radial gial cells.

Publikationen

Species	Application	Title
mouse	WB	ACS Nano; Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy; Authors - Qiqi Zhang
mouse	WB	Biomaterials; MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.; Authors - Mingming Song
human	IF	Nat Commun; Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.; Authors - Reuven Edri
mouse	IHC	Cancer Res; Bcl-xL enforces a slow-cycling state necessary for survival in the nutrient-deprived microenvironment of pancreatic cancer.; Authors - Yogev Sela
rat	WB	Int J Biol Macromol; Soybean soluble polysaccharide prevents obesity in high-fat diet-induced rats via lipid metabolism regulation; Authors - Haiyan He
mouse	IHC	Front Immunol; RGC-32 Regulates Generation of Reactive Astrocytes in Experimental Autoimmune Encephalomyelitis.; Authors - Alexandru Tatomir