FADD Polyklonaler Antikörper

FADD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(53)

Kat-Nr. : 14906-1-AP

Synonyme

MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHT-1080.Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Pankreasgewebe
Erfolgreiche IPA549-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Kolongewebe, Mausnierengewebe, Rattennierengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:2000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:500-1:2000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

14906-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA FADD und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Affe, Maus, Ratte, Rind
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen FADD fusion protein Ag6701
Vollständiger Name Fas (TNFRSF6)-associated via death domain
Berechnetes Molekulargewicht 23 kDa
Beobachtetes Molekulargewicht 23-30 kDa
GenBank-ZugangsnummerBC000334
Gene symbol FADD
Gene ID (NCBI) 8772
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for FADD antibody 14906-1-APProtokoll herunterladen
IHC protocol for FADD antibody 14906-1-APProtokoll herunterladenl
IF protocol for FADD antibody 14906-1-APProtokoll herunterladen
IP protocol for FADD antibody 14906-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Cell Res

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Authors - Jia-Yuan Zhang
human,mouseWB

Cell Commun Signal

Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury

Authors - Wenjing Sun
mouseWB

PLoS Biol

A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5.

Authors - Yingying Zhang
humanWB

Eur J Med Chem

PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism

Authors - Hongyan Lin
ratWB,IHC

Cancers (Basel)

Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1

Authors - Tiantian Qiu
humanWB,IP

Int Immunopharmacol

EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis

Authors - Huiling Zhang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Isha (Verified Customer) (12-15-2021)

Got really good bands in kidney lysates at 1:800 dilution

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:800
  • Cell Tissue Type: kidney