FADD Polyklonaler Antikörper
FADD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus und mehr (3)
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 14906-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HT-1080.Zellen, A549-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Pankreasgewebe |
Erfolgreiche IP | A549-Zellen |
Erfolgreiche Detektion in IHC | humanes Lungenkarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Kolongewebe, Mausnierengewebe, Rattennierengewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:500-1:2000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 1 publications below |
WB | See 48 publications below |
IHC | See 7 publications below |
IP | See 5 publications below |
CoIP | See 1 publications below |
Produktinformation
14906-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA FADD und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte, Rind |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | FADD fusion protein Ag6701 |
Vollständiger Name | Fas (TNFRSF6)-associated via death domain |
Berechnetes Molekulargewicht | 23 kDa |
Beobachtetes Molekulargewicht | 23-30 kDa |
GenBank-Zugangsnummer | BC000334 |
Gene symbol | FADD |
Gene ID (NCBI) | 8772 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
---|---|
WB protocol for FADD antibody 14906-1-AP | Protokoll herunterladen |
IHC protocol for FADD antibody 14906-1-AP | Protokoll herunterladenl |
IF protocol for FADD antibody 14906-1-AP | Protokoll herunterladen |
IP protocol for FADD antibody 14906-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
---|---|
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Cell Commun Signal Deubiquitinase USP5 regulates RIPK1 driven pyroptosis in response to myocardial ischemic reperfusion injury | ||
PLoS Biol A unique death pathway keeps RIPK1 D325A mutant mice in check at embryonic day 10.5. | ||
Eur J Med Chem PKM2/PDK1 dual-targeted shikonin derivatives restore the sensitivity of EGFR-mutated NSCLC cells to gefitinib by remodeling glucose metabolism | ||
Cancers (Basel) Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1 | ||
Int Immunopharmacol EGFR-TNFR1 pathway in endothelial cell facilitates acute lung injury by NF-κB/MAPK-mediated inflammation and RIP3-dependent necroptosis |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Isha (Verified Customer) (12-15-2021) | Got really good bands in kidney lysates at 1:800 dilution
|