FADD Polyklonaler Antikörper

FADD Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14906-1-PBS

Synonyme

FAS-associated death domain protein, FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, MORT1

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  WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 14906-1-AP (same clone as 14906-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 14906-1-AP (same clone as 14906-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

  mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 14906-1-AP (same clone as 14906-1-PBS)

  IP result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 14906-1-AP (same clone as 14906-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14906-1-AP (same clone as 14906-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (14906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14906-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA FADD und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FADD fusion protein Ag6701
• Vollständiger Name: Fas (TNFRSF6)-associated via death domain
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 23-30 kDa
• GenBank-Zugangsnummer: BC000334
• Gene symbol: FADD
• Gene ID (NCBI): 8772
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)