FADS2 Monoklonaler Antikörper

FADS2 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

CloneNo.

1G4B10

Kat-Nr. : 68026-1-Ig

Synonyme

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase, 1G4B10

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  WB analysis of PC-3 using 68026-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of LNCaP using 68026-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of U2OS using 68026-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 68026-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 68026-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 68026-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 68026-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 68026-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68026-1-Ig (FADS2-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 68026-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FADS2 antibody (68026-1-Ig, Clone: 1G4B10 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, A549-Zellen, Jurkat-Zellen, LNCaP-Zellen, Rattenlebergewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

68026-1-Ig bindet in WB, IHC, IF/ICC, ELISA FADS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FADS2 fusion protein Ag27715
• Vollständiger Name: fatty acid desaturase 2
• Berechnetes Molekulargewicht: 445 aa, 49 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC009011
• Gene symbol: FADS2
• Gene ID (NCBI): 9415
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.

Publikationen

Species	Application	Title
mouse	WB	Proteomics; Proteomics coupled transcriptomics reveals lipopolysaccharide inhibiting peroxisome proliferator-activated receptors signalling pathway to reduce lipid droplets accumulation in mouse liver; Authors - Haiying Ran
human	WB	Sci Rep; FADS2 confers SCD1 inhibition resistance to cancer cells by modulating the ER stress response; Authors - Toshikatsu Ikeda ; KD Validated
human	WB	FASEB J; Lysosomal membrane protein TMEM106B modulates hematopoietic stem and progenitor cell proliferation and differentiation by regulating LAMP2A stability; Authors - Di Guo
mouse	WB	Prostaglandins Leukot Essent Fatty Acids; Fatty acid synthase global inducible knockout does not alter brain fatty acid concentrations but attenuates cholesterol synthesis in the adult mouse; Authors - Drew R Seeger
rat	WB	Lipids; Exogenous oxidized phytosterol may modulate linoleic acid metabolism through upregulation of fatty acid desaturase in rats; Authors - Tomonari Koyama
human	WB	Mol Biol Rep; FADS2 inhibits colorectal cancer cell proliferation by regulating ferroptosis through SLC7A11/GPX4; Authors - Qinghui Yang