FADS2 Polyklonaler Antikörper

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• KD/KO Validated

FADS2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (2)

Kat-Nr. : 28034-1-AP

Synonyme

EC:1.14.19.3, Delta-6 desaturase, D6D, Acyl-CoA 6-desaturase

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• 

  WB analysis using 28034-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 28034-1-AP (FADS2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 28034-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 28034-1-AP (FADS2-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 28034-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 28034-1-AP (FADS2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 28034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28034-1-AP (FADS2 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 28034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28034-1-AP (FADS2 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Produktinformation

28034-1-AP bindet in WB, IHC, IF, ELISA FADS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FADS2 fusion protein Ag27709
• Vollständiger Name: fatty acid desaturase 2
• Berechnetes Molekulargewicht: 445 aa, 49 kDa
• Beobachtetes Molekulargewicht: 46-52 kDa
• GenBank-Zugangsnummer: BC009011
• Gene symbol: FADS2
• Gene ID (NCBI): 9415
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms and can be detected as 46-52 kDa.

Publikationen

Species	Application	Title
human	WB	Cell Chem Biol; FADS2-dependent fatty acid desaturation dictates cellular sensitivity to ferroptosis and permissiveness for hepatitis C virus replication.; Authors - Daisuke Yamane ; KD Validated
human	WB	FASEB J; PMID: 31199678; Authors - Ge Liu
human,mouse	WB,IF	Immun Inflamm Dis; Identification of Ferroptosis-Related Gene in Age-Related Macular Degeneration Using Machine Learning; Authors - Meijiang Zhu
	IF	BME Front; Multimodal Metabolic Imaging Reveals Pigment Reduction and Lipid Accumulation in Metastatic Melanoma; Authors - Hyeon Jeong Lee
mouse	WB	FASEB J; Comparing Soy and Milk Protein Regulation of Hepatic Omega-3 Fatty Acid Biosynthesis; Authors - Melissa Gonzalez-Soto
mouse	WB	Pharmacol Res; Breaking the synergism of iron overload and miR-122 to rescue lipid accumulation and peroxidation in MASLD; Authors - Yuxiao Tang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xianfeng (Verified Customer) (02-18-2020)

Works well with overexpressed mouse FADS2 but no signal with endogenouse mouse FADS2.

• Applications: Western Blot,

FADS2 Antibody Western Blot, validation ( dilution) in (Cat no:28034-1-AP)

FH

XIANFENG (Verified Customer) (02-06-2020)

Works well with overexpressed mouse FADS2, but does not work with endogenous mouse FADS2 (mouse liver) .

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000

FADS2 Antibody Western Blot, validation (1:1000 dilution) in (Cat no:28034-1-AP)