FADS2 Rekombinanter Antikörper

FADS2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241863D2

Kat-Nr. : 84527-4-RR

Synonyme

241863D2, Acyl-CoA 6-desaturase, D6D, Delta-6 desaturase, EC:1.14.19.3

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  WB analysis of L02 using 84527-4-RR

  L02 cells were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 84527-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84527-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84527-4-RR (FADS2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  Affinity and Kinetic Characterization of 84527-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84527-4-RR against Human FADS2-Specific were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen, A549-Zellen, Jurkat-Zellen, LNCaP-Zellen, Mauslebergewebe, PC-3-Zellen, Rattenlebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84527-4-RR bindet in WB, ELISA FADS2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: FADS2 fusion protein Ag27715
• Vollständiger Name: fatty acid desaturase 2
• Berechnetes Molekulargewicht: 445 aa, 49 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC009011
• Gene symbol: FADS2
• Gene ID (NCBI): 9415
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Fatty acid desaturase 2 (FADS2) is responsible for the first desaturation reaction in the synthesis of highly unsaturated fatty acids (HUFAs), such as arachidonic acid (20:4n-6) and eicosapentaenoic acid (20:5n-3), and is involved in Mead acid (20:3n-9) production during essential fatty acid deficiency (EFAD) (PMID: 29353041). This is important when temperatures changes and the membrane is under distress. It has 4 isoforms produced by alternative splicing.