FADS3 Polyklonaler Antikörper

FADS3 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15205-1-PBS

Synonyme

fatty acid desaturase 3, FADS 3, EC:1.14.19.-, Delta(13) fatty acid desaturase (Delta(13) desaturase ), Cytochrome b5 related protein

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  WB analysis using 15205-1-AP (same clone as 15205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15205-1-AP (same clone as 15205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 15205-1-AP (same clone as 15205-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 15205-1-AP (same clone as 15205-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15205-1-AP (FADS3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 15205-1-AP (same clone as 15205-1-PBS)

  IP result of anti-FADS3 (IP:15205-1-AP, 4ug; Detection:15205-1-AP 1:500) with L02 cells lysate 3000 ug. This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 15205-1-AP (same clone as 15205-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15205-1-AP (FADS3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15205-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15205-1-PBS bindet in WB, IHC, IP, Indirect ELISA FADS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FADS3 fusion protein Ag7355
• Vollständiger Name: fatty acid desaturase 3
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 41-51 kDa
• GenBank-Zugangsnummer: BC004901
• Gene symbol: FADS3
• Gene ID (NCBI): 3995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FADS3, also named as CYB5RP, belongs to fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family memebers are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase protion, both of which are characterized by conserved histidine motifs. FADS3 can be detected as 40 kDa-51 kDa.