FAF2 Polyklonaler Antikörper

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FAF2 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16251-1-PBS

Synonyme

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  WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16251-1-AP (same clone as 16251-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16251-1-AP (FAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16251-1-AP (same clone as 16251-1-PBS)

  WB result of FAF2 antibody (16251-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FAF2 transfected HeLa cells. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  IP result of anti-FAF2 (IP:16251-1-AP, 3ug; Detection:16251-1-AP 1:1000) with mouse brain tissue lysate 5000ug. This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 16251-1-AP (same clone as 16251-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16251-1-AP (FAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16251-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16251-1-PBS bindet in WB, IHC, IP, Indirect ELISA FAF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAF2 fusion protein Ag9236
• Vollständiger Name: Fas associated factor family member 2
• Berechnetes Molekulargewicht: 445 aa, 53 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC014001
• Gene symbol: FAF2
• Gene ID (NCBI): 23197
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protein ETEA (FAF2, or UBXD8) is a homolog to Fas-associated factor 1 (FAF1), which is involved in Fas-mediated apoptosis. ETEA protein directly interacts with and negatively regulates neurofibromin. ETEA contains both UBA and UBX domains and overexpression of ETEA downregulates neurofibromin in human cells. It may play a role in the translocation of terminally misfolded proteins from the endoplasmic reticulum lumen to the cytoplasm and their degradation by the proteasome. ETEA is highly expressed in peripheral blood of patients with atopic dermatitis (AD) compared to normal individuals, and may regulate the resistance to apoptosis that is observed in T cells and eosinophils of AD patients.