FAH Monoklonaler Antikörper

FAH Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F8E11

Kat-Nr. : 67986-1-PBS

Synonyme

2F8E11, Beta-diketonase, EC:3.7.1.2, FAA, Fumarylacetoacetate hydrolase

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  WB analysis using 67986-1-Ig (same clone as 67986-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 67986-1-Ig (same clone as 67986-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 67986-1-Ig (same clone as 67986-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 67986-1-Ig (same clone as 67986-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67986-1-Ig (FAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67986-1-Ig (same clone as 67986-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67986-1-Ig (same clone as 67986-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAH antibody (67986-1-Ig, Clone: 2F8E11 ) at dilution of 1:150 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 67986-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67986-1-PBS bindet in WB, IF/ICC, Indirect ELISA FAH und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: FAH fusion protein Ag6704
• Vollständiger Name: fumarylacetoacetate hydrolase (fumarylacetoacetase)
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC002527
• Gene symbol: FAH
• Gene ID (NCBI): 2184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAH is also named as FAA(fumarylacetoacetase), the last enzyme in tyrosine degradation, functionally important in the liver and kidney(PMID:8473520). FAH is a metabolic enzyme catalyzing the last step of tyrosine and phenylalanine catabolism: the hydrolysis of fumarylacetoacetate into acetoacetate and fumarate. In humans, deficiency of this activity is associated with the metabolic disease hereditary tyrosinaemia type I, which is also known as hepatorenal tyrosinaemia(PMID:17768357).