FAK Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

FAK Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12636-1-PBS

Synonyme

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

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  WB analysis using 12636-1-AP (same clone as 12636-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:70000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 12636-1-AP (same clone as 12636-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12636-1-AP (FAK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12636-1-AP (same clone as 12636-1-PBS)

  IP result of anti-FAK (IP:12636-1-AP, 4ug; Detection:12636-1-AP 1:30000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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  IHC staining of Hepatocellular carcinoma using 12636-1-AP (same clone as 12636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12636-1-AP (same clone as 12636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12636-1-AP (same clone as 12636-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12636-1-AP (FAK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 12636-1-AP (same clone as 12636-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FAK antibody (12636-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 12636-1-AP (same clone as 12636-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using FAK antibody (12636-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red). This data was developed using the same antibody clone with 12636-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12636-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA FAK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAK fusion protein Ag3331
• Vollständiger Name: PTK2 protein tyrosine kinase 2
• Berechnetes Molekulargewicht: 1052 aa, 119 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC028733
• Gene symbol: FAK
• Gene ID (NCBI): 5747
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.