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FAK Monoklonaler Antikörper

FAK Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2c

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(57)

CloneNo.

2C5B9

Kat-Nr. : 66258-1-Ig

Synonyme

PTK2, 2C5B9, EC:2.7.10.2, FADK 1, FAK1



Geprüfte Anwendungen

Erfolgreiche Detektion in WBhTERT-RPE1-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hodengewebe, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe, RAW 264.7-Zellen, ROS1728-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHUVEC-Zellen, A549-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66258-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA FAK und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG2c
Klonalität Monoklonal
Typ Antikörper
Immunogen FAK fusion protein Ag17966
Vollständiger Name PTK2 protein tyrosine kinase 2
Berechnetes Molekulargewicht 1052 aa, 119 kDa
Beobachtetes Molekulargewicht 110 kDa
GenBank-ZugangsnummerBC028733
Gene symbol FAK
Gene ID (NCBI) 5747
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for FAK antibody 66258-1-IgProtokoll herunterladen
IHC protocol for FAK antibody 66258-1-IgProtokoll herunterladenl
IF protocol for FAK antibody 66258-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Sci Adv

Lipid-anchored proteasomes control membrane protein homeostasis

Authors - Ruizhu Zhang
ratWB

Acta Biomater

Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury.

Authors - Shanshan Ma
humanWB

Cancer Lett

Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma

Authors - Qianyu Shi
humanWB

Oncogene

Rab34 regulates adhesion, migration, and invasion of breast cancer cells.

Authors - Lixiang Sun
humanWB

Oncogene

MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer.

Authors - Ran-Ran Ma
humanWB

Aging (Albany NY)

TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages.

Authors - Jiao Wu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Balawant (Verified Customer) (12-02-2022)

This antibody is working great both for mice colon sample as well as for colon epithelial cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mice colon tissue and caco2/HCT116 cells