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FAK Monoklonaler Antikörper
FAK Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA
Wirt / Isotyp
Maus / IgG2c
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IHC, IF/ICC, IP, CoIP, ELISA
Konjugation
Unkonjugiert
CloneNo.
2C5B9
Kat-Nr. : 66258-1-Ig
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | hTERT-RPE1-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, humanes Hodengewebe, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, Maushirngewebe, NIH/3T3-Zellen, Hausschwein-Hirngewebe, Kaninchenhirngewebe, Rattenhirngewebe, RAW 264.7-Zellen, ROS1728-Zellen |
| Erfolgreiche Detektion in IHC | humanes Mammakarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
| Erfolgreiche Detektion in IF/ICC | HUVEC-Zellen, A549-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunhistochemie (IHC) | IHC : 1:50-1:500 |
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 1 publications below |
| WB | See 44 publications below |
| IHC | See 4 publications below |
| IF | See 16 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Produktinformation
66258-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA FAK und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, Maus, Ratte |
| Wirt / Isotyp | Maus / IgG2c |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | FAK fusion protein Ag17966 |
| Vollständiger Name | PTK2 protein tyrosine kinase 2 |
| Berechnetes Molekulargewicht | 1052 aa, 119 kDa |
| Beobachtetes Molekulargewicht | 110 kDa |
| GenBank-Zugangsnummer | BC028733 |
| Gene symbol | FAK |
| Gene ID (NCBI) | 5747 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative promoter usage and alternative splicing, and the range of the molecular weights are 100-120kD and 40-60kD.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for FAK antibody 66258-1-Ig | Protokoll herunterladen |
| IHC protocol for FAK antibody 66258-1-Ig | Protokoll herunterladenl |
| IF protocol for FAK antibody 66258-1-Ig | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Acta Biomater Sodium alginate/collagen/stromal cell-derived factor-1 neural scaffold loaded with BMSCs promotes neurological function recovery after traumatic brain injury. | ||
Cancer Lett Direct contact between tumor cells and platelets initiates a FAK-dependent F3/TGF-β positive feedback loop that promotes tumor progression and EMT in osteosarcoma | ||
Oncogene MiR-19a/miR-96-mediated low expression of KIF26A suppresses metastasis by regulating FAK pathway in gastric cancer. | ||
Aging (Albany NY) TNFSF9 promotes metastasis of pancreatic cancer through Wnt/Snail signaling and M2 polarization of macrophages. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Balawant (Verified Customer) (12-02-2022) | This antibody is working great both for mice colon sample as well as for colon epithelial cells
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