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FAK Rekombinanter Antikörper

FAK Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242295E4

Kat-Nr. : 81334-5-PBS

Synonyme

PTK2, EC:2.7.10.2, FADK 1, FAK1, Focal adhesion kinase 1

Filter:
  • Western Blot (WB) analysis of various lysates using FAK Recombinant monoclonal antibody (81334-5-RR)

    WB analysis using 81334-5-RR (same clone as 81334-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81334-5-RR (FAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FAK Recombinant monoclonal antibody (81334-5-RR)

    IF Staining of A549 using 81334-5-RR (same clone as 81334-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FAK Recombinant monoclonal antibody (81334-5-RR)

    IF Staining of MCF-7 using 81334-5-RR (same clone as 81334-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FAK antibody (81334-5-RR, Clone: 242295E4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81334-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81334-5-RR

    Affinity and Kinetic Characterization of 81334-5-RR

    Biolayer interferometry (BLl) kinetic assays of 81334-5-RR against Human FAK were performed. The affinity constant is 2.03 nM.

Geprüfte Anwendungen

Produktinformation

81334-5-PBS bindet in WB, IF/ICC, Indirect ELISA FAK und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen FAK fusion protein Ag3331
Vollständiger Name PTK2 protein tyrosine kinase 2
Berechnetes Molekulargewicht 1052 aa, 119 kDa
Beobachtetes Molekulargewicht 110 kDa
GenBank-ZugangsnummerBC028733
Gene symbol FAK
Gene ID (NCBI) 5747
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971).