Phospho-FAK (Tyr397) Rekombinanter Antikörper

Phospho-FAK (Tyr397) Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240630F8

Kat-Nr. : 83933-1-PBS

Synonyme

FAK (Tyr397), p FAK, p FAK (Tyr397), p FAK Tyr397, p-FAK

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  WB analysis using 83933-1-RR (same clone as 83933-1-PBS)

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 83933-1-RR (Phospho-FAK (Tyr397) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-FAK (Tyr397) antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 83933-1-RR (same clone as 83933-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using Phospho-FAK (Tyr397) antibody (83933-1-RR, Clone: 240630F8 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83933-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83933-1-PBS bindet in WB, IF/ICC, ELISA Phospho-FAK (Tyr397) und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: PTK2 protein tyrosine kinase 2
• Berechnetes Molekulargewicht: 1052 aa, 119 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC028733
• Gene symbol: FAK
• Gene ID (NCBI): 5747
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative splicing and contains several phosphorylation and SUMOylation modification sites.