FAM127 Polyklonaler Antikörper

FAM127 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20282-1-PBS

Synonyme

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  WB analysis of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 20282-1-AP (same clone as 20282-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  IP experiment of PC-3 using 20282-1-AP (same clone as 20282-1-PBS)

  IP result of anti-FAM127 (IP:20282-1-AP, 4ug; Detection:20282-1-AP 1:500) with PC-3 cells lysate 3960 ug. This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 20282-1-AP (same clone as 20282-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 20282-1-AP (same clone as 20282-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using FAM127B antibody 20282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 20282-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20282-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA FAM127 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM127 fusion protein Ag13930
• Vollständiger Name: family with sequence similarity 127, member B
• Berechnetes Molekulargewicht: 113 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC000393
• Gene symbol: FAM127B
• Gene ID (NCBI): 26071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.