FAM127 Polyklonaler Antikörper

FAM127 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Rezensionen (1)

Kat-Nr. : 20282-1-AP

Synonyme

CXX1, CXX1b, CXX1C, FAM127, FAM127 A/B/C, MAR8, MAR8A, MAR8B, MAR8C, RTL8, RTL8 A/B/C, RTL8A, RTL8B, RTL8C

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  WB analysis of PC-3 using 20282-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20282-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20282-1-AP (FAM127B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of PC-3 using 20282-1-AP

  IP result of anti-FAM127 (IP:20282-1-AP, 4ug; Detection:20282-1-AP 1:500) with PC-3 cells lysate 3960 ug.

• 

  IHC staining of human colon using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20282-1-AP (FAM127B antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 20282-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 20282-1-AP (FAM127B antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 20282-1-AP

  Immunofluorescent analysis of HepG2 cells, using FAM127B antibody 20282-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: PC-3-Zellen, HeLa-Zellen
• Erfolgreiche IP: PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

20282-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA FAM127 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FAM127 fusion protein Ag13930
• Vollständiger Name: family with sequence similarity 127, member B
• Berechnetes Molekulargewicht: 113 aa, 13 kDa
• Beobachtetes Molekulargewicht: 13 kDa
• GenBank-Zugangsnummer: BC000393
• Gene symbol: FAM127B
• Gene ID (NCBI): 26071
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The FAM127 family currently has three members, namely FAM127A (also called RTL8C and CXX1), FAM127B (also called CXX1B and RTL8A), and FAM127C (also called RTL8B and CXX1C). They are located on chromosomes and their functions are unknown. All three are composed of 113 amino acids and have extremely high sequence similarity. The immunogen of this antibody has a sequence similarity of more than 95% with members of the FAM127 family. Theoretically, the antibody can recognize all members of the FAM127 family.

Publikationen

Species	Application	Title
human,mouse	WB	Cell Mol Life Sci; RTL8 promotes nuclear localization of UBQLN2 to subnuclear compartments associated with protein quality control.; Authors - Harihar Milaganur Mohan
	IP	J Biol Chem; Global proteomics of Ubqln2-based murine models of ALS.; Authors - Alexandra M Whiteley
human		bioRxiv; Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties; Authors - Harihar M Mohan
human	WB	PLoS One; The gag-like gene RTL8 antagonizes PEG10-mediated virus like particles; Authors - Will Campodonico

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Harihar (Verified Customer) (01-28-2024)

The antibody works well for Western blots and we have validated it in knockout cell lines. There is however a lot of high molecular weight background bands. It is not suitable for IF.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500 (WB)
• Cell Tissue Type: HEK293, HeLa, Kolf 2 IPSCs

FAM127 Antibody Western Blot validation (1:500 (WB) dilution) in HEK293, HeLa, Kolf 2 IPSCs (Cat no:20282-1-AP)